Induction of mesenchymal stem cell differentiation in collagen hydrogel for constructing three dimensional organ

胶原水凝胶诱导间充质干细胞分化构建三维器官

• 批准号: 22791318
• 负责人: KAWAI Norikazu
• 金额: $ 1.08万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791318/
• 关键词: 再生医療; 組織工学; 細胞・組織; 骨芽細胞; 間葉系幹細胞; 再生医学

We examined the usefulness of three-dimensional culture in collagen hydrogel for inducing differentiation of rat mesenchymal stem cells into osteoblasts compared with two-dimensional culture. In this culture method, the expression of Runx2 gene in early culture phase became higher compared with two-dimensional culture, which induced differentiation into osteoblasts and increased the expression of ostrix gene and the secretion of osteocalcin proteins associated with the maturation of osteoblasts. Finally the percentage of matured osteoblasts was increased. Three-dimensional culture in collagen hydrogel is useful for inducing differentiation of stem cells.

与二维培养相比，我们研究了胶原水凝胶三维培养在诱导大鼠骨髓间充质干细胞分化为成骨细胞中的作用。在这种培养方法中，Runx2基因在早期培养阶段的表达高于二维培养，从而诱导向成骨细胞分化，并增加Ostrix基因的表达和与成骨细胞成熟相关的骨钙素蛋白的分泌。成骨细胞成熟率增加。胶原蛋白水凝胶三维培养可用于干细胞的诱导分化。