Induction of mesenchymal stem cell differentiation in collagen hydrogel for constructing three dimensional organ

我们研究了三维培养在胶原水凝胶诱导大鼠间充质干细胞分化为成骨细胞相比，二维培养的有用性。在这种培养方法中，与二维培养相比，Runx 2基因在早期培养阶段的表达变得更高，从而诱导成骨细胞分化，并增加ostrix基因的表达和与成骨细胞成熟相关的骨钙素蛋白的分泌。最终成熟成骨细胞的百分比增加。胶原水凝胶中的三维培养可用于诱导干细胞分化。

We examined the usefulness of three-dimensional culture in collagen hydrogel for inducing differentiation of rat mesenchymal stem cells into osteoblasts compared with two-dimensional culture. In this culture method, the expression of Runx2 gene in early culture phase became higher compared with two-dimensional culture, which induced differentiation into osteoblasts and increased the expression of ostrix gene and the secretion of osteocalcin proteins associated with the maturation of osteoblasts. Finally the percentage of matured osteoblasts was increased. Three-dimensional culture in collagen hydrogel is useful for inducing differentiation of stem cells.