The expression of HDAC and its function in endometrial cancer

HDAC在子宫内膜癌中的表达及其功能

• 批准号: 22791525
• 负责人: SUZUKI Akihisa
• 金额: $ 2.08万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791525/
• 关键词: 子宮内膜癌; HDAC; 発現解析; 予後解析; 子宮内癌; 子官内膜癌

Over expression of histone deacetylases(HDACs) has been reported in various human malignancies, however, the expression of HDACs in endometrial tissue has not been fully understood. We examined the expression of HDAC1, HDAC2, and HDAC3 in normal and malignant endometrial tissues, and found that both HDACs were significantly up-regulated in endometrial cancer. In addition, the expression of HDAC2 was significantly increased in higher grade carcinomas and was correlated positively with the poor patient prognosis. The treatment of two HDAC inhibitors[ Trichostatin A(TSA), Apicidine(AP), and SAHA] decreased the cellular proliferation of all endometrial carcinoma cell lines examined via the up-regulation of p21 and down-regulations of cyclin A. Furthermore, the silencing of cyclin A conferred the anti-tumor activity of HDAC inhibitor. Therefore, increased HDAC2 expression is involved in the proliferation and aggressive behaviors of endometrial carcinoma, suggesting the possibility of HDAC inhibitor being a promising anti-cancer drug for endometrial carcinoma.

在各种人类恶性肿瘤中已经报道了组蛋白脱乙酰基酶（HDACS）的表达过高，但是，子宫内膜组织中HDAC的表达尚未完全了解。我们检查了正常和恶性子宫内膜组织中HDAC1，HDAC2和HDAC3的表达，发现这两个HDAC在子宫内膜癌中都显着上调。此外，高级癌中HDAC2的表达显着增加，并且与患者预后不良呈正相关。治疗两种HDAC抑制剂[Trichostatin A（TSA），阿picidine（AP）和Saha]降低了通过p21上调和细胞周期蛋白下调的所有子宫内膜癌细胞系的细胞增殖。因此，HDAC2表达增加参与子宫内膜癌的增殖和攻击行为，这表明HDAC抑制剂可能是子宫内膜癌的有希望的抗癌药物。

项目成果

Immunohistochemical detection of histone deacetylases in endometrial carcinoma : involvement of histon deacetylase 2 in the proliferation of endometrial carcinoma cells.

子宫内膜癌中组蛋白脱乙酰酶的免疫组织化学检测：组蛋白脱乙酰酶2参与子宫内膜癌细胞的增殖。

• 发表时间: 2010
• 期刊: Hum Pathol
• 影响因子: 3.3
• 作者: Fakhry H;Miyamoto T;Kashima H;Suzuki A;Ke H;Konishi I;Shiozawa T
• 通讯作者: Shiozawa T