Quantitative analysis and the investigation of the pneumococcal nasopharyngeal colonization in children.

儿童鼻咽部肺炎球菌定植情况的定量分析及调查。

• 批准号: 22791623
• 负责人: OGAMI Masashi
• 金额: $ 2.5万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791623/
• 关键词: 鼻科学; 肺炎球菌; 肺炎球菌共通抗原; イムノクロマトグラフィー法; 鼻咽腔; リアルタイムPCR

Streptococcus pneumonia(S. pneumoniae) colonized in nasopharynx of children is an important pathogenic bacteria in pediatric acute otitis media and rhinosinusitis. In this study, we performed quantitative analysis of nasopharyngeal S. pneumonia by using real-time PCR method. In the healthy children, the concentration of S. pneumoniae showed an inverse relation with the age. On the other hands, the concentration of S. pneumoniae in the children with upper respiratory infection was higher than the healthy children. Moreover, we revealed that a new rapid antigen test for detection of pneumococci in middle ear fluids and nasopharyngeal secretions can be an available tool for detection S. pneumoniae.

链球菌肺炎(S。儿童鼻咽部定植的肺炎菌（Pneumoniae）是小儿急性中耳炎和鼻窦炎的重要致病菌。本研究采用实时荧光定量PCR方法对鼻咽部肺炎链球菌进行定量分析。在健康儿童中，肺炎链球菌浓度与年龄呈反比关系。另一方面，上呼吸道感染儿童的肺炎链球菌浓度高于健康儿童。此外，我们揭示了一种新的用于检测中耳液和鼻咽分泌物中肺炎球菌的快速抗原试验可以成为检测肺炎链球菌的有效工具。

项目成果

Quantitative serotype determination of nasopharyngeal carriage of Strep tococcus pneumoniae

鼻咽部肺炎链球菌携带血清型的定量测定

• 发表时间: 2010
• 作者: Masashi Ogami;Muneki Hotomi;Noboru Yamanaka
• 通讯作者: Noboru Yamanaka

A Comparison of Conventional and Molecular Microbiology in Detecting Differences in Pneumococcal Colonization in Healthy Children and Children with Upper Respiratory Illness

传统微生物学和分子微生物学检测健康儿童和上呼吸道疾病儿童肺炎球菌定植差异的比较

• 发表时间: 2010
• 期刊: Eur J pediatr
• 影响因子: 3.6
• 作者: Ogami M;Hotomi M;Togawa A;Yamanaka N
• 通讯作者: Yamanaka N

小児鼻咽腔細菌叢における肺炎球菌のダイナミズムに関する研究

小儿鼻咽部肺炎球菌动态研究

• 发表时间: 2010
• 作者: 小上真史;保富宗城;戸川彰久;田村真司;山中昇
• 通讯作者: 山中昇