Intraocular Concentrations of Cytokines and Chemokines in Rhegmatogenous Retinal Detachment and the Effect of Intravitreal Triamcinolone Acetonide

目的：测定孔源性视网膜脱离（RRD）患者玻璃体内注射曲安奈德（IVTA）前后房水趋化因子水平。对19例连续行IVTA治疗的RRD患者的19只眼进行了研究。17只无视网膜血管病变的眼作为对照。在IVTA前（基线）和IVTA后1天测定房水中白细胞介素-6 （IL-6）、IL-8、eotaxin、G-CSF、GM-CSF、ifn - γ、IP-10、单核细胞趋化蛋白-1 （MCP-1）、巨噬细胞炎症蛋白-1 α (MIP-1a)、β (MIP-1b)、RANTES、tnf - α和血管内皮生长因子（VEGF）的水平。在基线时，在RRD眼的水中检测到IL-6、IL-8、IP-10、MCP-1、MIP-1b和VEGF，且RRD眼的水中IL-6、IL-8、IP-10、MCP-1、MIP-1b和VEGF显著高于对照组。IVTA后1 d， IP-10、MCP-1、MIP-1b水平均显著降低，且IP-10、MCP-1、MIP-1b的降低具有相关性。IVTA能够抑制RRD眼睛中IP-10、MCP-1和MIP-1b的水平。IP-10、MCP-1和MIP-1b在水中水平的变化相似，这些趋化因子可能是治疗RRD的潜在靶标分子。

To determine the aqueous humor levels of chemokines before and after an intravitreal injection of triamcinolone acetonide (IVTA)in eyes with rhegmatogenous retinal detachment (RRD).Nineteen eyes of 19 consecutive patients with RRD who underwent IVTA were studied. 17 eyes without retinal vascular disease served as control.The aqueous humor levels of interleukin-6 (IL-6), IL-8, eotaxin, G-CSF, GM-CSF, IFN-gamma, IP-10, monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1 alpha (MIP-1a), beta (MIP-1b), RANTES, TNF-alpha and vascular endothelial growth factor (VEGF)were determined before the IVTA (baseline)and at one day after the IVTA.At the baseline, IL-6, IL-8, IP-10, MCP-1, MIP-1b and VEGF were detected in the aqueous and were significantly higher in eyes with a RRD than in controls. One day after IVTA, the levels of IP-10, MCP-1 and MIP-1b were significantly reduced and the decreases in IP-10, MCP-1 and MIP-1b were correlated. IVTA was able to suppress the levels of IP-10, MCP-1 and MIP-1b in eyes with RRD. The alterations in the aqueous level of IP-10, MCP-1 and MIP-1b were similar and these chemokines might potentially be additional target molecules in therapy for RRD.