Development of vascularized artificial fat flap using adipose-derived stem cells

研究了脂肪源性干细胞（ASCs）在人工真皮、游离脂肪动静脉血管束和组织工程室（TEC）中血管化脂肪瓣上的作用。实验1为游离脂肪组和动静脉血管束组，实验2为血管化脂肪组。评估再生组织的数量，并使用1,1 '-二十八烷基- 3,3,3 ',3'-四甲基吲哚碳菁高氯酸盐（DiI）追踪ASCs。结果：再生血管化软组织体积为2。12±0。48和2。20±0。在实验1中，对照组和ASCs组分别为094 cm^3。30±0。19和1。36±0。实验2分别为22 cm^3，两种实验均无显著差异(p> 0。05)。组织病理学检查无明显差异。此外，在TEC再生软组织中未观察到dii标记细胞。这些结果表明，在TEC早期，ASCs已经坏死。

The effects of adipose-derived stem cells(ASCs) seeded on artificial dermis, arteriovenous vascular bundles with free fat, and a vascularized fat flap placed in a tissue engineering chamber(TEC) were investigated. The samples were divided into control and ASCs groups in Experiment 1 with free fat and arteriovenous vascular bundles, and Experiment 2 with vascularized fat. The amount of regenerated tissue was evaluated, and ASCs were traced using 1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine perchlorate(DiI).Results : The regenerated vascularized soft tissue volumes were 2. 12±0. 48 and 2. 20±0. 094 cm^3 in the control and ASCs groups in Experiment 1, respectively, and 1. 30±0. 19 and 1. 36±0. 22 cm^3 in Experiment 2, respectively, showing no significant difference in either experiment(p> 0. 05). No significant difference was also noted on histopathological examination. Moreover, no DiI-labeled cell was observed in the regenerated soft tissue in TEC. These findings suggest that ASCs were necrotized early in TEC.