Development of vascularized artificial fat flap using adipose-derived stem cells

使用脂肪干细胞开发血管化人造脂肪瓣

• 批准号: 22791724
• 负责人: HAMAMOTO Yusuke
• 金额: $ 1.66万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791724/
• 关键词: 脂肪由来幹細胞; 人工脂肪弁; 細胞外マトリックス; 再生組織; 人工皮弁; ティッシュエンジニアリングチャンバー; 脂肪弁; ティシューエンジニアリングチャンバー; FGF-2

The effects of adipose-derived stem cells(ASCs) seeded on artificial dermis, arteriovenous vascular bundles with free fat, and a vascularized fat flap placed in a tissue engineering chamber(TEC) were investigated. The samples were divided into control and ASCs groups in Experiment 1 with free fat and arteriovenous vascular bundles, and Experiment 2 with vascularized fat. The amount of regenerated tissue was evaluated, and ASCs were traced using 1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine perchlorate(DiI).Results : The regenerated vascularized soft tissue volumes were 2. 12±0. 48 and 2. 20±0. 094 cm^3 in the control and ASCs groups in Experiment 1, respectively, and 1. 30±0. 19 and 1. 36±0. 22 cm^3 in Experiment 2, respectively, showing no significant difference in either experiment(p> 0. 05). No significant difference was also noted on histopathological examination. Moreover, no DiI-labeled cell was observed in the regenerated soft tissue in TEC. These findings suggest that ASCs were necrotized early in TEC.

研究了脂肪衍生的干细胞（ASC）对人造真皮，带有游离脂肪的动静脉血管束的影响，以及放置在组织工程室（TEC）中的血管化脂肪皮瓣。在实验1中，将样品分为对照组和ASCS组，并用游离脂肪和动静脉血管束，并用血管化脂肪进行实验2。评估了再生组织的量，并使用1、1'-二二甲苯二烷基-3、3、3'，3'-四甲基丁基核酸苯胺高氯酸盐（DII）追踪ASC。再生：再生的血管化软组织体积为2。12±0。 48和2。20±0。实验1中的对照组和ASCS组中的094 cm^3和1。30±0。 19和1。36±0。实验2中的22 cm^3分别显示在任何一个实验中均无显着差异（p>0。05）。在组织病理学检查中也没有明显的差异。此外，在TEC的再生软组织中未观察到DII标记的细胞。这些发现表明，ASC在TEC早期被坏死。

项目成果

Tissue engineering chamber内の脂肪弁に与える脂肪由来幹細胞の影響

脂肪干细胞对组织工程室脂肪瓣的影响