Establishment of a method to promote osteoblast differentiation using tryptophan derivatives and Dex, and elucidation of the mechanisms of them

色氨酸衍生物和Dex促进成骨细胞分化的方法的建立及其机制的阐明

• 批准号: 22791778
• 负责人: MIKAMI Yoshikazu
• 金额: $ 2.5万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791778/
• 关键词: デキサメサゾン; トリプトファン誘導体; 骨芽細胞; 未分化間葉系幹細胞; 共培養; Osterix; 石灰化

In this study, we investigated the effect of newly synthesized tryptophan derivatives and dexamethasone (Dex), a synthetic glucocorticoid, on osteoblast differentiation. The tryptophan derivatives and Dex enhanced terminal osteoblast differentiation, as indicated by mineralization. However, no inductive effect was observed on commitment of mesenchymal stem cells (MSC) into osteoblast differentiation was observed. Furthermore, it was suggested that a transcription factor, Osterix, played an important role in the mechanism by which tryptophan derivatives and dexamethasone induced terminal osteoblast differentiation. In addition, we demonstrated that these agents significantly induced mRNA expression levels of the osteogenic marker genes bone sialoprotein (BSP) and Alkaline Phosphatase (ALP) in MSCs co-cultured with osteoblasts.

在这项研究中，我们研究了新合成的色氨酸衍生物和地塞米松（Dex），一种合成的糖皮质激素，对成骨细胞分化的影响。色氨酸衍生物和右美托咪唑增强末梢成骨细胞分化，矿化表明。然而，未观察到对间充质干细胞（MSC）向成骨细胞分化的诱导作用。此外，我们认为转录因子Osterix在色氨酸衍生物和地塞米松诱导终末成骨细胞分化的机制中发挥了重要作用。此外，我们证明这些药物显著诱导成骨标记基因骨涎蛋白（BSP）和碱性磷酸酶（ALP）在与成骨细胞共培养的MSCs中的mRNA表达水平。

项目成果

成熟骨芽細胞MLO-A5と未分化間葉系幹細胞C3H10T1/2の共培養系を用いた細胞間相互作用の検討.

使用成熟成骨细胞 MLO-A5 和未分化间充质干细胞 C3H10T1/2 的共培养系统检查细胞间相互作用。

• 发表时间: 2013
• 作者: 青山絵理子;久保田聡;滝川正春;三上剛和他.
• 通讯作者: 三上剛和他.