Analysis ofthe cell polarity in osteoclasts

破骨细胞不论处于何种分化阶段，均表达大量rabb家族GTPase和kif家族运动蛋白。我们使用GFP融合系统和sirna敲低系统分析了一些被认为具有极性的分子的定位变化。我们分析的所有分子都没有影响，这些结果似乎显示了rab家族蛋白质的冗余性。2、槲皮素是许多水果中发现的主要类黄酮之一，对破骨细胞的分化有抑制作用。使用特异性激动剂和拮抗剂的实验表明，槲皮素主要通过GPR30、gpcr型雌激素受体和Akt磷酸化途径发挥作用。

Many of Rab-family GTPase and KIF-family motor proteins were expressed in osteoclast regardless of its differentiation stage. We analyzed the changes of localization of some molecules that are thought to have polarity using GFP fusion system and siRNA-knockdown system. All of the molecules we analyzed have no effects, and these results seem to show the redundancy of Rab-family proteins. 2, Quercetin, one of major flavonoids found in many fruits, inhibited the differentiation of osteoclast. The assay using specific agonist and antagonist showed that the effects of quercetin were principally via GPR30, GPCR-type estrogen receptor, and Akt phosphorylation pathway.