Seasonal regulation of ion- and metabolite transport between poplar shoot tissues

杨树芽组织之间离子和代谢物运输的季节调节

• 批准号: 5398237
• 负责人: Dr. Peter Ache
• 金额: --
• 依托单位: Lehrstuhl für Botanik I: Molekulare Pflanzenphysiologie und Biophysik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5398237?language=en
• 关键词: Seasonal; regulation; ion; metabolite; transport

We intend to investigate the molecular mechanisms of mineral nutrient dependent poplar physiology with special focus on potassium. This will be accomplished using two different approaches. 1. Molecular biology: We will study the regulation of ion channels and transporters by different environmental conditions, such as the effect of nutrition, salt, hormonal action, cold and drought during wood production and the dormancy-growth transitions. Phenotype analysis of transporter sense/antisense plants will be used to gain insights into the role of the transporters in tree physiology. On the basis of a laser-micro-dissection system, we will be able to prepare cDNA of distinct cell types and generate subtractive cDNAs to determine genes, specific for the differentiation of vessels and bast fibers. 2. Electrophysiological investigations: We will compare the functional properties of the transporters. Ion-fluxes and transporters, involved in cambial activation will be characterized in vivo and in vitro. The response to changes in e.g. the extracellular medium in vitro, will provide a measure for the regulation of ion transport by apoplastic factors in vivo. Based on this data sets we should be able to establish a model on the seasonal fluxes of potassium in relation to the transporter properties and dynamics in the context of tree physiology in general and xylogenesis in particular.

本研究旨在探讨杨树矿质营养生理的分子机制，特别是钾的作用。这将使用两种不同的方法来实现。1.分子生物学：我们将研究不同环境条件对离子通道和转运蛋白的调节，例如营养，盐，激素作用，木材生产过程中的寒冷和干旱以及休眠-生长过渡的影响。表型分析的转运正义/反义植物将被用来深入了解的作用，运输树生理。在激光显微切割系统的基础上，我们将能够制备不同细胞类型的cDNA，并产生消减cDNA，以确定导管和韧皮纤维分化的特异性基因。2.电生理学研究：我们将比较转运蛋白的功能特性。参与形成层激活的离子通量和转运蛋白将在体内和体外进行表征。对例如体外细胞外介质中的变化的响应将提供体内通过质外体因子调节离子转运的措施。基于这些数据集，我们应该能够建立一个模型的季节性钾通量的运输性能和动态的背景下，一般的树木生理和特别是木质部。