Effects of Purified Bioactive Peptides on Bone Regeneration

纯化的生物活性肽对骨再生的影响

• 批准号: 22791840
• 负责人: YAMADA Shizuka
• 金额: $ 2.58万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791840/
• 关键词: 歯内療法学; フィッシュコラーゲンペプチド; コラーゲン翻訳後修飾; コラーゲン架橋; コラーゲン成熟; 基質石灰化; MC3T3-E1細胞; 飛行時間型質量分析法

Collagen is one of the most widely used biomaterials in the fields of tissue engineering, cosmetics and functional foods. Recently, fish collagen peptides(FCP) have been used as a dietary supplement, but its effects on cellular functions are poorly understood. In this study, the effects of FCP on the collagen synthesis and quality were investigated using an osteoblastic MC3T3-E1 cell culture system. When cells were treated with 0. 2%(w/ v) FCP, gene expression levels of collagen modifying enzymes, i. e. lysyl hydroxylases(LH1-3), lysyl oxidases(LOX and LOXL1-4) and glycosyltransferase 25 domain containing 1(GLT25D1) were all, except LOX and LOXL1, significantly upregulated at 48 hrs of culture. In addition, when collagen deposited at 14 days of culture was analyzed, the FCP treated group showed significantly higher collagen content, a greater extent of lysine hydroxylation(Hyl), higher levels of Hyl-aldehyde derived cross-links and accelerated cross-link maturation, in comparison to the untreated group. Furthermore, it was found that FCP treatment accelerated the matrix mineralization in the cultures. These results indicate that FCP exerts positive effects on collagen synthesis, collagen quality and mineralization in an osteoblastic cell culture system, suggesting the potential utility of FCP for bone tissue engineering.

胶原蛋白是在组织工程、化妆品和功能食品等领域应用最为广泛的生物材料之一。近年来，鱼类胶原蛋白肽（FCP）已被用作膳食补充剂，但其对细胞功能的影响尚不清楚。本研究采用成骨细胞MC3T3-E1培养系统，研究了FCP对胶原合成和质量的影响。当细胞用0。在2%(w/ v) FCP条件下，除LOX和LOXL1外，胶原修饰酶赖氨酸羟化酶（LH1-3）、赖氨酸氧化酶（LOX和LOXL1-4）和含1糖基转移酶25结构域（GLT25D1）的基因表达量在培养48 h时均显著上调。此外，对培养第14天沉积的胶原进行分析时，与未处理组相比，FCP处理组胶原含量显著增加，赖氨酸羟基化（Hyl）程度更大，Hyl-醛衍生交联水平更高，交联成熟速度加快。此外，还发现FCP处理加速了培养物的基质矿化。这些结果表明，FCP在成骨细胞培养系统中对胶原合成、胶原质量和矿化有积极的影响，表明FCP在骨组织工程中的潜在应用价值。