Histological examination of bone regeneration induced by biomaterials mingled with mesenchymal stem cells

我们检测了我们的细胞递送系统诱导的骨再生，多孔β - tcp块与骨髓基质细胞混合，以澄清该模型中骨再生过程中的组织学事件。从股骨中获得的骨髓基质细胞被诱导成成骨细胞样细胞，然后将其植入多孔的β - tcp块上。不含基质细胞的β - tcp块作为对照。将骨块植入大鼠颅骨，进行组织学检查。分化组新生骨在β - tcp块的外围，然后向β - tcp块的中心延伸。在对照组中，仅在β - tcp块的底部观察到少量新骨。因此，这些结果表明分化的骨髓基质细胞/ β - tcp复合材料具有很高的成骨潜力。

We examined bone regeneration induce by our cell-delivery system, a porous beta-TCP block mingled with bone marrow stromal cells, in order to clarify histological events during the bone regeneration in this model. Bone marrow stromal cells obtained from the femora were induced into osteoblast-like cells, and then, seeded on a porous beta-TCP blocks. Beta-TCP blocks without the stromal cells were used as a control. The blocks were implanted in the rat calvariae and examined histologically. In the differentiated group, new bone was observed in the periphery of beta-TCP blocks, and then, it extended to the center of the beta-TCP blocks. In the control group, only a little new bone was observed in the bottom of the beta-TCP blocks. Thus, these results suggest that differentiated bone marrow stromal cells/beta-TCP composites have high osteogenic potential.