Histological examination of bone regeneration induced by biomaterials mingled with mesenchymal stem cells

我们研究了由我们的细胞递送系统诱导的骨再生，该系统是一种与骨髓基质细胞混合的多孔β-TCP块，以澄清该模型中骨再生过程中的组织学事件。将骨髓基质细胞诱导为成骨样细胞，然后接种在多孔β-TCP块上。不含基质细胞的β-TCP块用作对照。将块植入大鼠颅骨中并进行组织学检查。在分化组中，在β-TCP块的周边观察到新骨，然后，它延伸到β-TCP块的中心。在对照组中，仅在β-TCP块的底部观察到少量新骨。因此，这些结果表明，分化的骨髓基质细胞/β-TCP复合材料具有高的成骨潜力。

We examined bone regeneration induce by our cell-delivery system, a porous beta-TCP block mingled with bone marrow stromal cells, in order to clarify histological events during the bone regeneration in this model. Bone marrow stromal cells obtained from the femora were induced into osteoblast-like cells, and then, seeded on a porous beta-TCP blocks. Beta-TCP blocks without the stromal cells were used as a control. The blocks were implanted in the rat calvariae and examined histologically. In the differentiated group, new bone was observed in the periphery of beta-TCP blocks, and then, it extended to the center of the beta-TCP blocks. In the control group, only a little new bone was observed in the bottom of the beta-TCP blocks. Thus, these results suggest that differentiated bone marrow stromal cells/beta-TCP composites have high osteogenic potential.