A study of the development high activity ex vivo produced oral mucosa equivalent by cryopreserved oral keratinocytes.

一项关于通过冷冻保存的口腔角质形成细胞开发高活性离体产生的口腔粘膜等效物的研究。

• 批准号: 22791964
• 负责人: KOYAMA Takahiro
• 金额: $ 2.58万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791964/
• 关键词: 組織工学; 口腔粘膜上皮前駆・幹細胞; 凍結保存; 凍結培養粘膜

The aim of this study was to development of high activity freeze culture cell mucosa.The cell activity of filtered keratinocytes compared these of not filtered keratinocytes. We made culture composition oral mucosa (freeze EVPOME) which we used the cell for and it was histological and immunohistological evaluated it in 4, culture mucosa of each group of the eleventh day after cell dissemination. A filtering (+) group compared cell count with a filtering (-) group and exceeded it in each time, and there was many it, and base cell count of freeze EVPOME, PCNA counts were found in a filtering (+) group after cell dissemination by the fourth day and the eleventh day. The results of this study were indicated that the freeze EVPOME of a filtering (+) group were higher activity than a thing of a filtering (-) group.

本研究旨在建立一种高活性的冷冻培养细胞粘膜，并将过滤后的角质形成细胞与未过滤的角质形成细胞进行细胞活性比较。我们制作了我们使用细胞的培养组合物口腔粘膜（冷冻EVPOME），并在细胞播散后第11天的各组培养粘膜中对其进行组织学和免疫组织学评价。滤过（+）组与滤过（-）组细胞计数比较，每次均超过滤过（-）组，且有较多的细胞数，滤过（+）组在细胞播散后第4天和第11天出现冷冻EVPOME的基础细胞计数、PCNA计数。本研究结果表明，过滤（+）组的冷冻EVPOME活性高于过滤（-）组。