An elucidated study of the healing process by used ex vivo produced oral mucosa equivalent by cryopreserved oral keratinocytes.

一项对使用冷冻保存的口腔角质形成细胞体外产生的口腔粘膜等效物的愈合过程进行的阐明性研究。

• 批准号: 19791499
• 负责人: KOYAMA Takahiro
• 金额: $ 2.42万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19791499/
• 关键词: 組織工学; 口腔粘膜上皮細胞; 凍結保存; 治癒機転; ラット移植モデル

The aim of this study was to elucidate the process of healing after transplantationed human ex vivo-produced oral mucosa equivalents by cryopreserved oral keratinocytes (frozen EVPOMEs). Normal EVPOMEs, by not cryopreserved oral keratinocytes, was used as a control. Two groups of EVPOMEs were transplanted into the back of nude mice. Mice were killed on days 1, 7, 14, 21, and 28 posttransplantation. The healing process of the transplanted EVPOMEs was evaluated with histologically. There were differences of the vessel density and of the epithelial stratification between two groups on 14 days posttransplantation. However, There were no difference between two groups on 28 days posttransplantation. The results of this study were indicated that two groups of EVPOMEs was same process of healing posttransplantation.

本研究的目的是阐明冷冻保存的口腔角质形成细胞(EVPOME)移植人体外产生的口腔黏膜等效物后的愈合过程。正常EVPOME未经冷冻保存的口腔角质形成细胞作为对照。将两组EVPOME移植到裸鼠背部。分别于移植后第1、7、14、21、28天处死小鼠。组织学观察移植的EVPOME的愈合过程。移植后14d，两组血管密度和上皮复层差异有统计学意义。但在移植后28天，两组间差异无统计学意义。本研究结果表明，两组EVPOME移植后愈合过程相同。