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Analysis of structural abnormality of whole chromosomes and novel tumor suppressor gene using mapping arrays in oral cancer

利用映射芯片分析口腔癌全染色体结构异常和新型抑癌基因

基本信息

批准号：
22792017
负责人：
YAMAMOTO Nobuharu
金额：
$ 2.5万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22792017/
关键词：
口腔がん DNAマッピングアレイヘテロ接合性消失(LOH)口腔癌

项目摘要

[Purpose] We analyzed genomic copy number abnormality on all genomes in the oral squamous cell carcinoma using Affymetrix GeneChip^<(R)> Mapping 10K Array in this study and tried the identification of the new metastasis connection gene of the oral cavity squamous cell carcinoma. [Materials and Methods] We used 20 oral squamous cell carcinoma cases. We analyzed a metastasis to regional lymph node-positive case(pN(+)) 3 case and a negative case(pN(-)) 2 case by GeneChip(R) Mapping 10K Array for histopathology and detected genome copy number abnormalitywere common to a pN(+) case as comparedwith a pN(-) case, and tobefound and listed the meta(we) stasis connection gene candidates who were present in theatrical company rank. Furthermore, we inspected the mRNA manifestation situation of the candidate gene by quantitative Real-time PCR.[Results] In the region that we were common to a pN(+) case, and a genomic copy number increased, 16 regions, region that decreased were 32 regions. We listed 75 existing candidate genes on these regions. A ZDHHC14 gene did locus in the one 6q25.3 region of the region that a copy number specifically increased for a pN(+) case. As a result of having assayed the mRNA expression level of the gene, high-frequency manifestation sthenia was found (40% of 8/20 cases). Furthermore, the mRNA manifestation sthenia was significantly found in a pN(+) case (P=0.019).[Conclusions] These results suggested the possibility that ZDHHC14 was a new metastasis of the oral squamous cell carcinoma-related gene. As for this study, information as we exceeded imagination with the conducted genomic analysis much more was obtained till now, and the results were given as a new search method surely and this gave a presentation by foreign countries thesis and gave large result.

【目的】本研究应用AffysseGeneChip ^<（R）> Mapping 10 K Array分析口腔鳞癌全基因组拷贝数异常，并尝试寻找口腔鳞癌转移相关的新基因。[材料与方法]收集20例口腔鳞癌患者。我们用GeneChip（R）Mapping 10 K Array分析了1例区域淋巴结转移阳性病例（pN（+））3例和1例区域淋巴结转移阴性病例（pN（-））2例的组织病理学，检测到pN（+）病例与pN（-）病例相比基因组拷贝数异常是共同的，并发现和列出了在剧院公司排名中存在的Meta（we）stasis连接候选基因。并通过实时荧光定量PCR检测候选基因的mRNA表达情况。[结果]在pN（+）病例共有的区域中，基因组拷贝数增加的区域为16个，减少的区域为32个。我们在这些区域上列出了75个现有的候选基因。ZDHHC 14基因在pN（+）病例中拷贝数特异性增加的区域的一个6q25.3区域中没有位点。检测该基因的mRNA表达水平，结果发现高频率的表现实（8/20例的40%）。pN（+）例mRNA表达增强（P=0.019）。[结论] ZDHHC 14可能是一个新的口腔鳞癌转移相关基因。关于这项研究，我们通过进行基因组分析获得了超出想象的信息，到目前为止，结果肯定是作为一种新的搜索方法给出的，这是国外论文的介绍，并给出了大量结果。