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Elucidation of the structural abnormality on all chromosomes using mapping array in oral cancer

使用口腔癌映射阵列阐明所有染色体上的结构异常

基本信息

批准号：
20791549
负责人：
YAMAMOTO Nobuharu
金额：
$ 2.58万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

项目摘要

[Purpose] We analyzed genomic copy number abnormality on all genomes in the squamous cell carcinoma of the tongue using Affymetrix GeneChipR Mapping 10K Array in this study and tried the identification of the new metastasis connection gene of the squamous cell carcinoma of the tongue. [Materials and a method] We used 20 squamous cell carcinoma of the tongue cases. We analyzed a metastasis to regional lymph node-positive case (pN(+)) 3 cases and a negative case (pN(-)) 2 cases by GeneChipR Mapping 10K Array for histopathology and detected genome copy number abnormality we were common to a pN(+) case as compared with a pN(-) case, and to be found and listed the metastasis connection gene candidates who were present in theatrical company rank. Furthermore, we inspected the mRNA manifestation situation of the candidate gene by quantitative Real-time PCR. [Results] In the region that we were common to a pN(+) case, and a genomic copy number increased, 16 regions, region that decreased were 32 regions. We listed 75 existing candidate genes on these regions. A ZDHHC14 gene did locus in the one 6q25.3 region of the region that a copy number specifically increased for a pN(+) case. As a result of having assayed the mRNA expression level of the gene, high-frequency manifestation sthenia was found (40% of 8/20 cases). Furthermore, the mRNA manifestation sthenia was significantly found in a pN(+) case (P = 0.019). [Conclusions] These results suggested the possibility that ZDHHC14 was a new metastasis of the squamous cell carcinoma of the tongue-related gene.

【目的】本研究应用AffysseGeneChipR Mapping 10 K Array分析舌鳞癌全基因组拷贝数异常，并尝试寻找新的舌鳞癌转移相关基因。[材料与方法]收集20例舌鳞状细胞癌病例。我们通过GeneChipR Mapping 10 K Array分析了3例转移到区域淋巴结阳性病例（pN（+））和2例转移到区域淋巴结阴性病例（pN（-））的组织病理学，并检测了pN（+）病例与pN（-）病例相比常见的基因组拷贝数异常，并发现并列出了在剧院公司排名中存在的转移相关候选基因。并通过实时荧光定量PCR检测候选基因的mRNA表达情况。[结果]在pN（+）病例共有的区域中，基因组拷贝数增加的区域为16个，减少的区域为32个。我们在这些区域上列出了75个现有的候选基因。ZDHHC 14基因在pN（+）病例中拷贝数特异性增加的区域的一个6q25.3区域中没有位点。检测该基因的mRNA表达水平，结果发现高频率的表现实（8/20例的40%）。pN（+）例mRNA表达增强（P = 0.019）。[结论] ZDHHC 14可能是一个新的舌鳞癌转移相关基因。