Molecular mechanisms of condroclastgenesis in temporomandibular joint. osteoarthrosis ; clarification of the hypoxia-inducible factor-1

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Giving mechanical stress, the cells were cultured under 1%, 5%, 10%, 15% pO2, and normoxic condition and HIF-1 activity was analyzed. As a result, HIF-1 was observed only under 1% pO2 condition without loading. On the other hand, HIF-1 tended to increase under every condition with loading. Furthermore the activation of HIF-1 was evaluated by a real-time RT-PCR analysis for HIF-1 target genes and osteoclast-relating genes. As a result, mechanical stimuli strikingly increased these genes expression under 1% and 5% pO2.