Long term cryopreservation of bone tissue for clinical use

温度骤降至-30℃，保持时间为-5℃15分钟，温度为0。1mt的磁场导致最大的成活率和生长率。ALP、OPN和BSP mRNA的表达量和蛋白浓度与对照组无显著差异。8周、12周和24周对照组均有新骨形成和骨桥。在冷冻保存和干燥4周和8周的组中，完整骨周围没有新骨形成。冷冻保存12周时观察到新骨形成，冷冻保存24周时观察到移植骨与完整骨连接。在12周和24周时，干燥组移植区有大量骨吸收。这些结果表明，骨组织冷冻保存可以成功地用于骨移植，这可能是颅骨成形术的一种新选择。

A plunging temperature of-30℃, a hold-time at-5℃for 15 minutes and a 0. 1 mT of a magnetic field led to both the greatest survival and growth rate. Moreover, there was no significant difference in ALP, OPN and BSP mRNA expression and protein concentration between cryopreserved and control groups. New bone formation and bone bridge were detected in 8, 12 and 24-week control groups. In 4-and 8-week cryopreserved and dried groups, there was no new bone formation around the intact bones. New bone formation was observed in 12-week cryopreserved group and the transplanted bone was connected to the intact bone in 24-week cryopreserved group. In 12 and 24 weeks, a large amount of bone resorption in the transplanted area was observed in the dried group. These results suggest that bone tissue cryopreservation can be successfully used for bone grafting which may be a novel option for cranioplasty.