Development of a new antimicrobial agent therapy to planperiodontal pocket reduction

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We investigated the effects of AZM on human gingival fibroblasts (hGFs) and human periodontal ligament cells (hPDLFs) by measuring the expression levels of the inflammatory cytokines IL-6 and IL-8, as well as MMP-1, and TIMP-1 and which are associated with connective tissue remodeling.The mRNA expression of IL-6 and IL-8 in hGFs was upregulated by AZM dose-dependently under LPS stimulation conditions. However, hPDLFs showed no association between AZM and IL-6 or IL-8. The hGFs upregulated MMP-1 and TIMP-1 in response to AZM dose-dependently.In addition, the hPDLFs did not exhibit differences in expression of MMP-1, MMP-2, TIMP-1, or TIMP-2.We speculate that such highly concentrated AZM would be able to supress periodontal pathogenic bacteria, destroy an already formed biofilms and periodontal pocket reduction in short time period.