Development of Microchip Electrophoresis.Mass Spectrometry Analysis of Biomolecules Using Nanoparticles

本研究将纳米粒子分别应用于对映体和磷酸肽的毛细管电泳和毛细管电色谱分析。为了进行对映体的CEC分析，采用离子交换法制备了辛可尼定（CCND）纳米晶，并将其固定在熔融石英毛细管和微芯片的内表面上。利用该纳米晶固定相成功地实现了外消旋氨基酸的CEC对映体分离。另一方面，在磷酸肽的CE分析中，开发了使用部分注入的二氧化钛纳米颗粒的在线预富集技术以提高检测灵敏度。通过向分离柱中注入一小段二氧化钛分散液，可以很好地浓缩从β-酪蛋白中释放出来的ATP和磷酸肽

In this study, nanoparticles were applied to capillary electrophoresis (CE) and capillary electrochromatography (CEC) analysis of enantiomers and phosphopeptides, respectively. For the CEC analysis of enantiomers, cinchonidine (CCND) nanocrystals were prepared by an emusion method to immobilize onto the inner surface of fused silica capillaries and microchips. By using the CCND nanocrystals stationary phase, the CEC enantioseparations of racemic amino acids were successfully achieved. In the CE analysis of phosphopeptides, on the other hand, an on-line preconcentration technique using partially injected titania nanoparticles was developed to improve the detection sensitivity. By injecting a short plug of the titania dispersion into the separation column, ATP and phosphopeptide released from ss-casein were well concentrated