Real-time monitoring of variable viscoelasticity of proteins during translation reaction

我们使用石英晶体微天平技术实时监测mRNA的翻译速率。这种方法使我们能够评估链亲和素结合肽融合蛋白上游融合的感兴趣蛋白的翻译。结果表明，翻译速率不仅受mRNA序列的调控，还受翻译蛋白的调控。通过评价蛋白质在翻译过程中的粘弹性，并监测翻译速率，我们将能够了解被翻译蛋白质的性质如何影响翻译反应。

We used a quartz crystal microbalance technique to monitor the rate of mRNA translation in real time. This method permitted us to evaluate the translation of proteins of interest fused upstream of a streptavidin-binding peptide fusion protein. The results indicated that the rate of translation can be regulated by not only the sequence of mRNA, but also the protein translated. We will be able to understand how the property of the protein translated affects the reaction of translation by evaluating the viscoelasticity of the protein during translation together with monitoring the rate of translation.