Analysis of the aberrant translational stall and its release during translation reaction.

DNA上的遗传信息被转录成mRNA，然后被翻译成蛋白质。在翻译过程中，蛋白质的合成速率受多种因素的影响。因此，我们认为翻译反应可能出现异常失速并从失速中重新开始。在本研究中，我们建立了利用石英晶体微天平监测异常平移失速及其释放的方法。因此，我们发现翻译失速的频率取决于每个基因序列。此外，根据人工平移失速后的平移失速重启实验结果，提出了一种新的平移失速重启机制。

Genetic information on DNA is transcribed into mRNA and then translated into protein. Duding translation process, the rate of synthesis of protein was varied by various factors. Thus, it has been supposed that the translation reaction possibly stalls aberrantly and restarts from stalling. In this study, we established the methodology by using quart-rystal microbalance to monitor the aberrant translational stall and its release. As a result, we found that the frequency of translational stall depends on each sequence of genes. Moreover, we proposed a novel mechanism of translational stall and restart from the experimental results of translational restart from the artificially translational stall.