Development of the artificial transcription factor which promotes endogenous gene expression in cell

研制了扳手酚类似物，该类似物具有较好的水溶性和较低的分子量。合成了报道的具有转录激活能力的化合物10，并利用链霉亲和素-生物素系统对该化合物进行了转录激活试验。结果表明，通过该系统，化合物10没有表现出转录激活能力。采用糖皮质激素受体-地塞米松系统测定化合物10的转录激活能力。通过该系统，化合物10也没有表现出转录激活能力。

The wrenchnolol analogue was developed, it has improved water solubility and with lower molecule weight.The compound 10 which reported have transcriptional activation ability was synthesized, and this compound was assayed by transcriptional activation assay using streptavidin-biotin system. As the result, by this system, the compound 10 did not show transcriptional activation ability.The transcriptional activation ability of the compound 10 was measured using glucocorticoid receptor-dexamethasone system. By this system, the compound 10 also did not show transcriptional activation ability.