Cell-free synthesis and in vitro reconstitution of cellulase/hemicellulases supramolecular complex

嗜热厌氧菌热梭菌产生的纤维素酶/半纤维素酶超分子复合体(纤维素体)对结晶纤维素具有较高的糖化活性。在本研究中，我们分析了由无细胞合成的纤维素体成分在体外重组的超分子纤维素体的糖化活性。结果表明，结晶纤维素的糖化活性随着支架蛋白中纤维体结合域(粘附素)数量的增加而增加。此外，我们还成功地从堆肥超基因组中分离到四个粘附素基因。

Cellulase/hemicellulases supramolecular complex(cellulosome) from thermophilic anaerobe Clostridium thermocellum shows a high saccharification activity for crystalline cellulose. In this study, we analyzed the saccharification activity of the C. thermocellum supramolecular cellulosome, which is in vitro reconstituted from cell-free synthesized cellulosomal components. As a result, it was revealed that the saccharification activity for crystalline cellulose increased along with an increase in the number of cellulosome-binding domains(cohesins) in the scaffolding protein. Furthermore, we successfully isolated four cohesin genes from a compost metagenome.