Analyses of functional RNAs and target genes involves in leg regeneration using transgenic cricket, Gryllus bimaculatus

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We cloned several miRNAs from libraries of embryonic and regenerating tissues and observed these expressions in the embryos. We revealed expression patterns of cloned miRNAs. We found miR-8, which is a target of Wnt component gene in our miRNAs libraries. Moreover, to analyze the function of miRNA and its target genes, we established cell trajectory analysis using the transgenic cricket. Furthermore, we established gene-targeting systems using zinc finger nucleases(ZFNs) and transcription activator-like(TAL) effector nucleases(TALENs). Using these systems, we are now trying to investigate the function of the miRNA involves in the leg regeneration in cricket.