Structure of photoreceptor membrane protein by In-situ photoirradiated solid-state NMR
Structure of photoreceptor membrane protein by In-situ photoirradiated solid-state NMR
批准号:
22770101
负责人:
KAWAMURA Izuru
金额:
$2.66万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
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英文摘要
In situ photoirradiated solid-state NMR have been developed and demonstrated to successfully observe the M-photointermediate of pharaonis phoborhodopsin(ppR) and T204A mutant in the lipid environment. The C^<13> NMR signals of[20-C^<13>] retinal-ppR with transducer revealed that multiple M-photointermediates with 13-cis, 15-anti retinal configuration under continuously photoirradiated condition. Our results provide insights into the process of photocycle in the ppR with transducer protein.
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固体NMRによるアミロイド.膜タンパク質の構造解析
通过固态 NMR 进行淀粉样蛋白膜蛋白的结构分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[I. Kawamura, R. Furusato, T. Kondo, T. Okitsu, A. Wada, N. Kamo, A. Naito, 川村出]
通讯作者:
川村出
An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy
原位光照射固态核磁共振光谱揭示了一种活性光感受器中间体
DOI:
10.1016/j.bpj.2011.10.022
发表时间:
2011
期刊:
Biophys.J.
影响因子:
--
作者:
[Tomonaga, Y., Hidaka, T, Kawamura, I, Nishio, T, Ohsawa, K, Okitsu, T, Wada, A, Sudo, Y, Kamo, N, Ramamoorthy, A, Naito, A.]
通讯作者:
A.
DOI:
10.1002/anie.201004422
发表时间:
2011-02
期刊:
Angewandte Chemie
影响因子:
--
作者:
[Lichi Shi;I. Kawamura;K. Jung;L. Brown;V. Ladizhansky]
通讯作者:
Lichi Shi;I. Kawamura;K. Jung;L. Brown;V. Ladizhansky
Three-dimensional Solid-state NMR study of Anabaena Sensory Rhodopsin in the lipid environment-Chemical Shift Assignments-
脂质环境中鱼腥藻感觉视紫红质的三维固态核磁共振研究-化学位移分配-
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[I.Kawamura, L.Shi, L.S.Brown, V.Ladizhansky]
通讯作者:
V.Ladizhansky
Solid-state NMR structural study of anabaena sensory rhodopsin in the lipid environment
脂质环境中鱼腥藻感觉视紫红质的固态核磁共振结构研究
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[I. Kawamura, L. Shi, K. H. Jung, L. S. Brown, V. Ladizhansky]
通讯作者:
V. Ladizhansky
共 12 条
Conformational change of photoreceptor membrane protein using by in-situ photo-irradiated solid-state NMR
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批准号:24570127
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.49万
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财政年份:2012
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负责人:KAWAMURA Izuru
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依托单位:
Structural analysis by solid-state NMR with photo-illumination system
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批准号:20770080
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.08万
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财政年份:2008
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负责人:KAWAMURA Izuru
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依托单位:
海外基金