Development of the assay system for chromatin regulatory factors using modified centromere DNA

在细胞增殖过程中，染色体，我们的DNA信息的组成部分，需要复制和分离到每个分裂的子细胞，正确。一种名为着丝粒的功能性DNA/蛋白质复合物负责染色体分离。然而，其机制，即着丝粒结构是如何组织的，还不清楚。然后，我们构建了含有特异性蛋白结合位点的人工着丝粒DNA序列，并通过该结合位点连接了几种染色质相关的候选蛋白。结果发现，一种蛋白质修饰酶--组蛋白乙酰转移酶，可以诱导着丝粒DNA序列上的着丝粒从头形成。

During the cell proliferation, chromosomes, integral of our DNA information, are needed to be replicated and segregated to each divided daughter cell, correctly. A functional DNA/protein complex named centromere is responsible for chromosome segregation. However, the mechanisms, how centromere structure is organized, is yet unknown. Then, we created the artificial centromere DNA sequence containing a specific protein binding site, and tethered several kinds of chromatin related protein candidates through this binding site. As a result, we found that a kind of protein modification enzyme, histone acetyltransferase, can induce de novo centroemre formation on the centromere DNA sequence.