A study of molecular mechanisms of assembly of the nuclear envelope-like membrane using DNA-beads incorporated into living cells

本研究旨在探讨利用dna微珠在活细胞内组装核包膜样膜的分子机制。我们利用荧光显微镜和电子显微镜分析了各种细胞成分在dna小珠上组装的时间，发现在内体断裂后，小珠上出现了自整合障碍因子（BAF）。用RNAi去除BAF显著减少了珠子上核包膜蛋白的组装。基于这些结果，我们得出结论，BAF是dna珠依赖的NE组装的关键因素。

This research aims to investigate molecular mechanisms of assembly of the nuclear envelope(NE)-like membrane by utilizing DNA-beads incorporated into a living cell. We analyzed the timing of assembly of various cellular components on the DNA-beads using fluorescence microscopy and electron microscopy, and found that barrier-to-autointegration factor(BAF) appeared on the beads at very early timing after endosomal breakage. Removal of BAF with RNAi significantly reduced assembly of a nuclear envelope protein on the beads. Based on these results, we conclude that BAF is a critical factor for DNA-beads dependent NE assembly.