Moleclar genetic analysis of eukaryotic molybdate transport systems in a model yeast Hansenula polymorpha and a model animal cell

点击翻译按钮获取中文摘要

Molybdenum (Mo) is an essential trace element for almost all living organisms. Whilst living cells are known to import inorganic molybdate oxyanion from the surrounding environment, the in vivo dynamics of cytosolic molybdate and/or transport systems remain poorly understood. We investigated molybdate transport system of a model unicellular yeast Hansenula polymorpha in this study. Results of genetic analysis suggested the yeast has at least three defferent type of molybdate transport. Also, we obtained three candidate genes for the molybdate transport those exhibited molybdate accumulation in heterologous expression systems. Furthermore, we developed a genetically encoded Forester-resonance-energy-transfer (FRET)-based nanosensor for molybdate. By using the nanosensor we demonstrated in vivo dynamics of molybdate in a model animal cell HEK293T . Analyses of the dynamics suggest that novel oxalate-sensitive- and sulfate-resistant- transporter(s) uptake molybdate in thecell.