Biosynthetic study and its application of daurichromenic acid, an anti-HIV natural product

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In this study, I cloned genes encoding polyketide synthase and oxidocyclase that are involved in the biosynthetic pathway of daurichromenic acid, an anti-HIV component in Rhododendron dauricum, by means of RT-PCR, and characterized bacterially expressed recombinant polyketide synthase and oxidocyclase to clarify the structural and functional properties in detail. In addition, I attempted to obtain a gene for the prenyltransferase(farnesyltransferase) that synthesizes the carbon skeleton of daurichromenic acid by EST analysis, resulting in cloning of several candidate genes for the prenyltransferase. Interestingly, one of these putative prenyltransferases showed sequence similarity with a farnesyltransferase(protoheme farnesyltransferase).