Cloning and application of cannabinoid biosynthetic genes based on EST analysis

基于EST分析的大麻素生物合成基因克隆及应用

• 批准号: 20780084
• 负责人: TAURA Futoshi
• 金额: $ 2.83万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780084/
• 关键词: 生合成; EST; カンナビノイド; 遺伝子クローニング

In this study, I cloned a gene encoding oli vetol synthase, the polyketide synthase that synthesizes the phenolic moiety of cannabi noids in Cannabis sativa, by means of EST analysis and RT-PCR, and then characterized the bacterially expressed recombinant oli vetol synthase to clarify the structural and functional properties in detail. In addition, I attempted to obtaina gene for the prenyltransferase that synthesize the carbon skeleton of cannabinoids, resultingincloning of two candi date genes for the prenyltransferase. Interestingly, I confirmed that these putativeprenyltransferases showmore that 50% identity with the prenyltransferase involved in secondary net abolismin hop, a close relative of.C. sative.

本研究利用EST分析和RT-PCR技术克隆了大麻中合成大麻酚类物质的聚酮合酶基因，并对该基因进行了结构和功能的研究。此外，我还试图获得一个合成大麻素碳骨架的异戊烯基转移酶基因，结果克隆了两个异戊烯基转移酶的候选基因。有趣的是，我证实了这些推定的异戊二烯基转移酶与参与次级净代谢蛋白啤酒花的异戊二烯基转移酶有50%以上的同一性，啤酒花是C.满足感。

项目成果

Grystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa.

大麻聚酮合酶 1 (PKS-1) 的结晶和初步 X 射线衍射研究。

• 发表时间: 2008
• 期刊: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 64
• 作者: C. Taguchi;F. Tatra;T. Tanada;Y. Shoyana;Y. Shoyana;H. Tanaka;R. Kiroki;S. Morinoto
• 通讯作者: S. Morinoto

Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway

• DOI: 10.1016/j.febslet.2009.05.024
• 发表时间: 2009-06-18
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Taura, Futoshi;Tanaka, Shinji;Morimoto, Satoshi
• 通讯作者: Morimoto, Satoshi

Cannabinoid in Nature and Medicine (DM Iambert (Ed. ))

自然界和医学中的大麻素（DM Iambert（主编））

• 发表时间: 2009
• 作者: F. Taura;S. Sirikantaramas;Y. Shoyama;Y. Shoyama;S. Morimoto
• 通讯作者: S. Morimoto

Crystallization and preliminary X-ray diffraction studies of polyketide synthase from Cannabis sativa

大麻聚酮合酶的结晶和初步 X 射线衍射研究

• 发表时间: 2008
• 期刊: Acta Cryst., F 64
• 作者: Taguchi;C.;Taura;F.;et.al.
• 通讯作者: et.al.

Molecular characterization of the biosynthetic enzyme for the biotechnol ogical production of tetrahydrocannabinol, the active constituent of mari juana

用于生物技术生产大麻活性成分四氢大麻酚的生物合成酶的分子表征

• 发表时间: 2008
• 期刊: Drug Discoveries and Therapeutics 2, Suppl
• 作者: Taura;F.
• 通讯作者: F.