Exploring population-specific adaptive genes in the non-model fish species : comprehensive gene expression and population genomic analyses

为了检测自然种群中引起适应性分化的候选基因，以雌雄对虾虎鱼（Leucopsarion petersii）为模型系统，研究了一种非模式物种的综合基因表达分化筛选方法。利用基于aflp的基因表达谱方法“高覆盖表达谱（high coverage expression profiling, HiCEP）”获得的有限转录组谱在日本海和太平洋种群之间进行了比较。一些基因在这些群体之间表现出显著而稳定的表达差异。因此，本研究明确了HiCEP是探索基因组信息不可用的非模态物种基因表达差异的有用方法。

To detect candidate genes that caused adaptive divergence in natural populations, a screening method of comprehensive gene expression divergence for the non-model species was investigated using the anadromous goby Leucopsarion petersii as a model system. Limited transcriptome profiles obtained by an AFLP-based gene expression profiling method called"high coverage expression profiling(HiCEP)"were compared between the Japan Sea and Pacific Ocean populations of this species. Some genes showed striking and stable expression differences between these populations. Thus, this study clarified that HiCEP is a useful method for exploring gene expression divergence in the nonmode l species whose genomic information is not available.