Functional analysis of nucleoporin Tmem48/Ndc1 during gametogenesis

核孔蛋白在配子体发生过程中的功能尚不清楚，尽管在体外，核孔蛋白在多种生物学性质中发挥着重要作用，如核-细胞质运输和核形态组装。本研究旨在阐明核孔蛋白在配子体发生中的作用。结果表明，Tmem48/Ndc1结合了一些在生精细胞中特异性表达的蛋白，在小鼠胚胎成纤维细胞或生殖细胞减数分裂前期维持其核结构，对小鼠胚胎成纤维细胞的增殖活性是必不可少的。

The function of the nucleoporin during gametogenesis remains unknown, although the nucleoporin has an important role in various biological natures, such as the nucleo-cytoplasmic transport and nuclear morphology assembly, in vitro. In this study, it is proposed to elucidate the role of the nucleoporin during gametogenesis. As result, the examinations indicated that Tmem48/Ndc1 binds to some proteins which are specific expression in spermatogenic cells, serves the maintenance of nuclear architecture of the mouse embryonic fibroblast cells or the germ cells during meiosis prophase I, and is dispensable to the proliferation activity of the mouse embryonic fibroblast cells.