Development and application of a method for efficient gene transfer of non-viral DNA targeted to murine liver

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In this study, we attempted to improve hydrodynamics-based gene delivery (HGD) to achieve a highly efficient gene delivery that is targeted to the murine liver and tested whether the liver-specific expression of a gene of interest is possible. We demonstrated that upon performing HGD, mixing of plasmid DNA with polyethylenimine (PEI)-based reagent yielded best transfection efficiency in hepatocytes. With this improved technique, we succeeded in performing Cre-loxP-mediated gene switching in liver and to show lines of direct evidence for liver specific transgene expression.