Basic Research on Materialization of Apple Polyphenol as Functional Food

苹果多酚功能食品物化的基础研究

基本信息

批准号：
12794007
负责人：
NAKAMURA Shingo
金额：
$ 9.34万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for University and Society Collaboration
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Biological activities of apple polyphenol oligomer derived from unripe apple in this study. Peroxidation of low density lipoprotein was strongly inhibited by apple polyphenol oligomer in in vitro assay and its potency was equal to that of tea catechin. Dietary apple polyphenol oligomer lowered the levels of liver phospholipids hydroperoxides in rats in the dose-dependent manner. Dietary apple polyphenol oligomer also lowered the activities of hepatic HMG-CoA reductase and Δ6 desaturase in rats. On the other hand, the activity of hepatic cholesterol 7α-hydroxylase was increased by dietary apple polyphenol oligomer. Reflecting these modulations of enzyme activities, liver cholesterol level and Δ6 desaturation indices of tissue lipids were lowered and fecal excretion level of acidic steroids was increased in rats fed apple polyphenol oligomer. The ratio of HDL-cholesterol/total cholesterol was increased by intake of apple polyphenol oligomer The dimer and trimer of apple polyphenol oligomer were identified in serum in rats fed apple polyphenol oligomer. Moreover, many unidentified compounds derived from apple polyphenol oligomer were found in serum in rats fed apple polyphenol, although these compounds were not identified in serum in rats fed polyphenol-free diet. Therefore, absorbed and decomposed compounds derived from apple polyphenol oligomer may exhibit various biological functions such as antioxidative action and regulation of lipid metabolism.

在这项研究中，苹果多酚低聚物的生物学活性。在体外评估中，苹果多酚低聚物强烈抑制低密度脂蛋白的过氧化，其效力等于茶儿茶素。饮食中的苹果多酚低聚物以剂量依赖性的方式降低了大鼠中肝磷脂的水平。饮食中的苹果多酚低聚物还降低了大鼠中肝HMG-COA还原酶和Δ6去饱和酶的活性。另一方面，饮食中苹果多酚低聚物增加了肝胆固醇7α-羟化酶的活性。反映这些酶活性的这些调节，组织脂质的肝胆固醇水平和Δ6去饱和指数的降低，并且在饲养苹果多酚低聚物的大鼠中增加了粪便的极端酸性类固醇水平。苹果多酚低聚物的摄入增加了HDL-胆固醇/总胆固醇的比率增加了苹果多酚低聚物的二聚体和三聚体，在喂食苹果多酚低聚物的大鼠的血清中鉴定出来。此外，在喂养苹果多酚的大鼠的血清中发现了许多从苹果多酚低聚物中得出的未鉴定化合物，尽管这些化合物在喂养无多酚饮食的大鼠中未鉴定出这些化合物。因此，源自苹果多酚低聚物的吸收和分解化合物可能存在各种生物学功能，例如抗氧化作用和脂质代谢的调节。