Biomarker discovery in non-alcoholic steatohepatitis model mice

非酒精性脂肪性肝炎(NASH)是近年来公认的一种代谢综合征相关疾病，是导致肝硬变和癌症的重要危险因素。在经济发达的国家，NASH患者的数量正在迅速增加。由于NASH的分子发病机制尚未明确，目前对NASH的准确诊断和有效治疗尚无定论。为了克服这种情况，我们通过使用NASH模型小鼠、CDAA喂养的小鼠或TSOD小鼠来寻找疾病相关蛋白。采用双向差示凝胶电泳法(2D-DGE)比较NASH模型小鼠和正常小鼠肝组织蛋白质组的差异。从凝胶中回收差异表达的蛋白质，用质谱仪进行鉴定。对这些蛋白的进一步分析将有助于阐明NASH发生的分子机制，并开发有前景的诊断和治疗方法。

Non-alcoholic steatohepatitis(NASH) is recently recognized as a metabolic syndrome-related disease and proved to be an important risk factor for liver cirrhosis and cancer. Number of patient with NASH is rapidly increasing in economically advanced countries. Since molecular pathogenesis of NASH has not been cleared, precise diagnosis and effective therapy for NASH has not been established yet. To overcome such a situation, we searched disease-related proteins by using NASH model mice, CDAA-fed mice or TSOD mice. Proteome of liver tissues of NASH model mice and normal mice were compared by 2-dimentional differential gel electrophoresis(2D-DIGE). Proteins of differential expression were recovered from the gel and then identified by mass spectrometer. Further analysis of these proteins would lead to elucidation of molecular mechanism of development of NASH and development of promising diagnosis and therapeutic methods.