Biomarker discovery in non-alcoholic steatohepatitis model mice

非酒精性脂肪性肝炎模型小鼠生物标志物的发现

• 批准号: 22790104
• 负责人: TSUNODA Shin-ichi
• 金额: $ 2.5万
• 依托单位: 独立行政法人医薬基盤研究所
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790104/
• 关键词: 分子生物学; プロテオミクス

Non-alcoholic steatohepatitis(NASH) is recently recognized as a metabolic syndrome-related disease and proved to be an important risk factor for liver cirrhosis and cancer. Number of patient with NASH is rapidly increasing in economically advanced countries. Since molecular pathogenesis of NASH has not been cleared, precise diagnosis and effective therapy for NASH has not been established yet. To overcome such a situation, we searched disease-related proteins by using NASH model mice, CDAA-fed mice or TSOD mice. Proteome of liver tissues of NASH model mice and normal mice were compared by 2-dimentional differential gel electrophoresis(2D-DIGE). Proteins of differential expression were recovered from the gel and then identified by mass spectrometer. Further analysis of these proteins would lead to elucidation of molecular mechanism of development of NASH and development of promising diagnosis and therapeutic methods.

非酒精性脂肪性肝炎（NASH）最近被认为是一种代谢综合征相关疾病，并被证明是肝硬化和癌症的重要危险因素。在经济发达国家，NASH 患者数量正在迅速增加。由于NASH的分子发病机制尚未明确，NASH的精准诊断和有效治疗尚未建立。为了克服这种情况，我们通过使用NASH模型小鼠、CDAA喂养小鼠或TSOD小鼠来寻找与疾病相关的蛋白质。采用二维微分凝胶电泳（2D-DIGE）比较NASH模型小鼠与正常小鼠肝组织的蛋白质组。从凝胶中回收差异表达的蛋白质，然后通过质谱仪进行鉴定。对这些蛋白质的进一步分析将有助于阐明 NASH 发生的分子机制，并开发有前景的诊断和治疗方法。

项目成果

Proteomics-based analysis of non-alcoholic fatty liver disease (NAFLD) in mice

基于蛋白质组学的小鼠非酒精性脂肪肝病 (NAFLD) 分析

• 发表时间: 2010
• 作者: Watanabe T.;Yamashita T.;Nagano K.;Kanasaki S.;Yoshikawa T.;Yoshioka Y.;Itoh N.;Abe Y.;Kamada H.;Tsutsumi Y.;Tsunoda S.
• 通讯作者: Tsunoda S.

Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung.

• DOI: 10.1016/j.bbrc.2011.01.077
• 发表时间: 2011-02
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Y. Yoshida;T. Yamashita;K. Nagano;S. Imai;H. Nabeshi;T. Yoshikawa;Y. Yoshioka;Y. Abe;H. Kamada;Y. Tsutsumi;S. Tsunoda

抗体プロテオミクス技術による創薬ターゲットタンパク質の効率的探索

利用抗体蛋白质组学技术高效搜索药物发现靶蛋白

• 发表时间: 2011
• 作者: Imai S.;Nagano K.;Yoshida Y.;Okamura T.;Yamashita T.;Abe Y.;Yoshikawa T.;Yoshioka Y.;Kamada H.;Mukai Y.;Nakagawa S.;Tsutsumi Y.;Tsunoda S.;梅原崇史;角田慎一
• 通讯作者: 角田慎一

Development of a novel antibody proteomics system using a phage antibody library for efficient screening of tumor-related biomarker proteins.

使用噬菌体抗体库开发新型抗体蛋白质组学系统，用于有效筛选肿瘤相关生物标志物蛋白质。

• 发表时间: 2011
• 期刊: Biomaterials 32(1)
• 作者: Imai S.;Nagano K.;Yoshida Y.;Okamura T.;Yamashita T.;Abe Y.;Yoshikawa T.;Yoshioka Y.;Kamada H.;Mukai Y.;Nakagawa S.;Tsutsumi Y.;Tsunoda S.
• 通讯作者: Tsunoda S.