Development of imaging technique for D-serine dynamics in central nervous system

已知D-丝氨酸调节中枢神经系统(CNS)的突触功能。然而，D-丝氨酸在中枢神经系统中的时空动态尚不清楚。在这项研究中，我们的目标是开发一种新型的荧光探针来显示D-丝氨酸的动力学。为此，我们建立了高通量的筛选体系，用于荧光探针的研制。利用这一技术平台，我们获得了几种D-丝氨酸探针的候选荧光偶联物，它们有望在D-丝氨酸结合时显示出较大的荧光变化。

D-serine is known to regulate synaptic functions in a central nervous systems(CNS). However, spatiotemporal dynamics of D-serine in CNS remains unclear. In this study, we aimed to develop a novel fluorescent probes for visualizing D-serine dynamics. For this purpose, we established high throughput screening system for the development of fluorescent probe. By using this technical platform, we obtained several candidate fluorescent conjugates for D-serine probes which are expected to show a large fluorescence change upon D-serine binding.