Analyses of intercellular signaling during generation of induced pluripotent stem cell from somatic cells

微阵列分析显示，体细胞诱导多能干细胞生成过程中，许多参与细胞间信号转导的基因表达水平发生了变化。这些基因包括NF-kB信号的多种下游靶点。为了研究NF-kB信号在iPS细胞生成过程中的功能，我们进行了功能缺失实验，证明NF-kB信号对重编程过程有积极作用，但对已建立的iPS细胞的维持没有积极作用。

Microarray analysis showed the expression level of many genes involved in intercellular signaling changed during generation of induced pluripotent stem(iPS) cells from somatic cells. The genes included diverse downstream targets of NF-kB signal. To address the function of NF-kB signaling during iPS cell generation process, we conducted loss-of-function experiment and demonstrated that NF-kB signaling have positive effect on the reprogramming process, but not maintenance of established iPS cells.