Development of the evaluation method for individual iPS cells

强迫表达一组转录因子诱导体细胞成为多能干细胞（iPS细胞）。它们的形态、分化能力和基因表达谱几乎完全等同于胚胎干细胞。为了将iPS细胞技术应用于再生医学，需要稳定地生成高质量的iPS细胞。为了实现这一目标，需要高精度的评估方法来评估生成的iPS细胞的分化能力和肿瘤发生风险。在本研究中，我们在单细胞水平上进行了基因表达分析，并开发了一种新的iPS细胞质量评价方法。

Forced expression of a set of transcription factors induces somatic cells into pluripotent stem cells(iPS cells). Their morphology, differentiation capacity and gene expression profile are almost exactly equivalent to those in embryonic stem(ES) cells. In order to apply iPS cell technology in regenerative medicine, high-quality iPS-cells should be stably generated. To achieve this, high accuracy evaluation methods are needed to assess the differentiation capacity and tumorigenesis risk in generated iPS cells. In this study, we performed gene expression analysis at the single cell level and developed the novel evaluation method for iPS cells quality.