Synaptic formation and maturation regulated by membrane trafficking leading to higher function of the nervous system

膜运输调节突触的形成和成熟，从而提高神经系统的功能

• 批准号: 22790293
• 负责人: SAKANE Ayuko
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790293/
• 关键词: 脳・神経系形成; 高次神経機能獲得; シナプス形成・成熟; 小胞輸送; Rabファミリー低分子量G蛋白質; 脳・神経系

Rab family small G proteins serve as molecular switches in the regulation of vesicular trafficking. In this study, we focus on the role and mode of action of Rab3A, Rab13, and their related proteins in synaptic formation and maturation, leading to higher function of the nervous system. As to Rab3A, we found the mice lacking Rabconnectin, the Rab3A-related protein, exhibit neuronal dysfunction, probably because of misformation of synaptic organization. We also found that the Rab13-JRAB system regulates the reorganization of actin cytoskeleton during neurite extension in PC12 cells. Our results suggest that this system may simultaneously coordinate vesicle transport and actin cytoskeletal reorganization in synaptic formation followed by maturation.

Rab家族小G蛋白在调节囊泡运输中充当分子开关。在这项研究中，我们专注于Rab 3A，Rab 13及其相关蛋白在突触形成和成熟中的作用和作用方式，从而导致神经系统的更高功能。对于Rab 3A，我们发现缺乏Rab 3A相关蛋白Rabconnectin的小鼠表现出神经元功能障碍，可能是由于突触组织的畸形。我们还发现Rab 13-JRAB系统在PC 12细胞突起延伸过程中调节肌动蛋白细胞骨架的重组。我们的研究结果表明，该系统可以同时协调囊泡运输和肌动蛋白细胞骨架重组突触形成成熟。

项目成果

Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells.

• DOI: 10.1093/jb/mvs056
• 发表时间: 2012-07
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: Kanchanamala Withanage;K. Nakagawa;Mitsunobu Ikeda;H. Kurihara;Takumi Kudo;Zeyu Yang;A. Sakane;Takuya Sasaki;Y. Hata

細胞接着・運動においてRab13-JRAB系が制御する小胞輸送とアクチン細胞骨格再編成

细胞粘附和运动过程中 Rab13-JRAB 系统调控的囊泡运输和肌动蛋白细胞骨架重组

• 发表时间: 2011
• 作者: 坂根亜由子;佐々木卓也
• 通讯作者: 佐々木卓也

Rab13 Regulates Neurite Outgrowth in PC12 Cells through Its Effector Protein, JRAB/MICAL-L2

• DOI: 10.1128/mcb.01067-09
• 发表时间: 2010-02-15
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Sakane, Ayuko;Honda, Kazufumi;Sasaki, Takuya
• 通讯作者: Sasaki, Takuya