Role of selectin-carbohydrate interactions in rolling of cells in blood vessels of the brain

据报道，骨髓来源的细胞外渗到阿尔茨海默病模型小鼠的脑中。选择素和它们的碳水化合物配体之间的分子相互作用是白细胞通过血液渗透到组织中的第一步。在这里，我们研究了选择素及其配体在骨髓来源的细胞向阿尔茨海默病脑渗出中的可能参与。我们在小鼠骨髓细胞系中检测了选择素配体sialyl刘易斯X的细胞表面表达。我们还发现，老年阿尔茨海默病模型小鼠脑血管中的选择素的表达上调。我们已经证明，选择素配体参与阿尔茨海默病模型小鼠脑中骨髓细胞的滚动和粘附。这些结果表明，选择素-糖配体相互作用可能是骨髓源性细胞向阿尔茨海默病脑内外渗的重要因素。

It has been reported that bone marrow derived cells extravasate into the brain of Alzheimer model mice. Molecular interactions between selectins and their carbohydrate ligands are the first step of leukocyte infiltration into tissues through the blood. Here, we investigated possible involvement of selectins and their ligands in diapedesis of bone marrow derived cells into Alzheimer brain. We have detected cell surface expression of sialyl Lewis X, a ligand of selectins, ina mouse bone marrow cell line. We also found that expression of one of selectins was up-regulated in brain vasculature of aged Alzheimer model mice. We have shown that the selectin ligand was involved in rolling and adhesion of bone marrow cells in the brain of Alzheimer model mice. These results indicated that selectin-carbohydrate ligandinteraction might be important for extravasation of bone marrow derived cells into Alzheimer brain.