Role of selectin-carbohydrate interactions in rolling of cells in blood vessels of the brain

选择素-碳水化合物相互作用在脑血管细胞滚动中的作用

• 批准号: 22790303
• 负责人: UCHIMURA Kenji
• 金额: $ 2.5万
• 依托单位: Nagoya University (2011)National Center for Geriatrics and Gerontology (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790303/
• 关键词: 細胞・組織; 生体分子; 糖鎖; 脳・神経

It has been reported that bone marrow derived cells extravasate into the brain of Alzheimer model mice. Molecular interactions between selectins and their carbohydrate ligands are the first step of leukocyte infiltration into tissues through the blood. Here, we investigated possible involvement of selectins and their ligands in diapedesis of bone marrow derived cells into Alzheimer brain. We have detected cell surface expression of sialyl Lewis X, a ligand of selectins, ina mouse bone marrow cell line. We also found that expression of one of selectins was up-regulated in brain vasculature of aged Alzheimer model mice. We have shown that the selectin ligand was involved in rolling and adhesion of bone marrow cells in the brain of Alzheimer model mice. These results indicated that selectin-carbohydrate ligandinteraction might be important for extravasation of bone marrow derived cells into Alzheimer brain.

据报道，骨髓来源的细胞外渗到阿尔茨海默病模型小鼠的脑中。选择素和它们的碳水化合物配体之间的分子相互作用是白细胞通过血液渗透到组织中的第一步。在这里，我们研究了选择素及其配体在骨髓来源的细胞向阿尔茨海默病脑渗出中的可能参与。我们在小鼠骨髓细胞系中检测了选择素配体sialyl刘易斯X的细胞表面表达。我们还发现，老年阿尔茨海默病模型小鼠脑血管中的选择素的表达上调。我们已经证明，选择素配体参与阿尔茨海默病模型小鼠脑中骨髓细胞的滚动和粘附。这些结果表明，选择素-糖配体相互作用可能是骨髓源性细胞向阿尔茨海默病脑内外渗的重要因素。

项目成果

Use of a phage display antibody to measure the enzymatic activity of the Sulfs.

使用噬菌体展示抗体来测量 Sulf 的酶活性。

• DOI: 10.1016/s0076-6879(10)80003-5
• 发表时间: 2010
• 期刊: Methods in enzymology
• 作者: Uchimura,Kenji;Lemjabbar-Alaoui,Hassan;vanKuppevelt,ToinH;Rosen,StevenD
• 通讯作者: Rosen,StevenD

Cahbohydrate-protein interactions in the vasculature

脉管系统中碳水化合物-蛋白质相互作用

• 发表时间: 2011
• 作者: Ueno N;Taketomi Y;Yamamoto K;Hirabayashi T;Kamei D;Kita Y;Shimizu T;Shinzawa K;Tsujimoto Y;Ikeda K;Taguchi R;Murakami M;Kenji Uchimura
• 通讯作者: Kenji Uchimura

The Sulf-degrading heparan sulfate epitope accumulates in cerebral amyloid β plaques of mouse models and patients of Alzheimer's disease

硫降解硫酸乙酰肝素表位在小鼠模型和阿尔茨海默病患者的大脑β淀粉样蛋白斑块中积累

• 发表时间: 2010
• 作者: Hosono T;Hossain M;Britschgi M;Akatsu H;van Kuppevelt T;Michikawa M,Wyss-Coray T;Uchimura K.
• 通讯作者: Uchimura K.

Heparan Sulfate Subdomains that are Degraded by Sulf Accumulate in Cerebral Amyloid β Plaques of Alzheimer's Disease Evidence from Mouse Models and Patients

• DOI: 10.1016/j.ajpath.2012.01.015
• 发表时间: 2012-05-01
• 期刊: AMERICAN JOURNAL OF PATHOLOGY
• 影响因子: 6
• 作者: Hosono-Fukao, Tomomi;Ohtake-Niimi, Shiori;Uchimura, Kenji
• 通讯作者: Uchimura, Kenji

Lipoprotein lipase is a novel Abeta-binding protein that promotes glycosaminoglycan-dependent cellular uptake of Abeta in astrocytes

脂蛋白脂肪酶是一种新型 Abeta 结合蛋白，可促进星形胶质细胞中糖胺聚糖依赖性细胞摄取 Abeta

• 发表时间: 2011
• 期刊: J. Biol. Chem
• 作者: Nishitsuji;K.;Hosono;T.;Uchimura;K. and Michikawa;M
• 通讯作者: M