Role of selectin-carbohydrate interactions in rolling of cells in blood vessels of the brain

据报道，骨髓来源的细胞外渗到阿尔茨海默病模型小鼠的大脑中。选择素与其碳水化合物配体之间的分子相互作用是白细胞通过血液渗入组织的第一步。在这里，我们研究了选择素及其配体可能参与骨髓来源细胞向阿尔茨海默病大脑的迁移。我们检测了选择素配体sialyl Lewis X在小鼠骨髓细胞系中的细胞表面表达。我们还发现其中一种选择素在老年阿尔茨海默模型小鼠的脑血管系统中表达上调。我们已经证明选择素配体参与了阿尔茨海默病模型小鼠大脑中骨髓细胞的滚动和粘附。这些结果表明，选择-碳水化合物配体的相互作用可能是骨髓来源细胞外渗到阿尔茨海默病脑的重要原因。

It has been reported that bone marrow derived cells extravasate into the brain of Alzheimer model mice. Molecular interactions between selectins and their carbohydrate ligands are the first step of leukocyte infiltration into tissues through the blood. Here, we investigated possible involvement of selectins and their ligands in diapedesis of bone marrow derived cells into Alzheimer brain. We have detected cell surface expression of sialyl Lewis X, a ligand of selectins, ina mouse bone marrow cell line. We also found that expression of one of selectins was up-regulated in brain vasculature of aged Alzheimer model mice. We have shown that the selectin ligand was involved in rolling and adhesion of bone marrow cells in the brain of Alzheimer model mice. These results indicated that selectin-carbohydrate ligandinteraction might be important for extravasation of bone marrow derived cells into Alzheimer brain.