Epigenetic transcriptional master regulator for hematopoietic cell differentiation

转录因子PU。1是造血系统的主要调节因子，对髓细胞和b细胞谱系的发育至关重要。聚氨酯。1与其他转录因子和辅因子协同作用。如前所示，PU。1与作为辅激活因子的p300/cbp和作为辅抑制因子的HDAC相互作用。在本研究中，确定是否改性PU。1负责转换其在共激活因子和共抑制因子之间的关联，我们研究了乙酰化是否调节PU的物理和功能活性。1.我们发现PU。1在体内被乙酰化，当假定的ETS结构域乙酰化基序发生突变时，其抑制因子活性降低。我们的数据表明乙酰化可能调节红细胞的生物学功能。

Transcription factor PU. 1 is a hematopoietic master regulator and essential for the development of myeloid and B-cell lineages. PU. 1 functions in concert with other transcription factors and cofactors. As we shown previously represented, PU. 1 interact with both p300/cbp, which function as a coactivator and HDAC, which function as a corepressor. In this study, to determine whether modification of PU. 1 is responsible for switching its association between coactivaor and corepressor, we examined whether acetylation regulates the physical and functional activities of PU. 1.We found that PU. 1 was acetylated in vivo and its repressor activity was reduced when the putative acetylation motif in ETS domain were mutated. Our data suggest that acetylation might regulate the biological functions in erythroid cells.