Transcriptional regulation of PU.1, which is a master regulator of hematopoietic cell differentiation

转录因子PU.1是一种造血主调节因子，对髓系细胞和B细胞系的发育至关重要。PU1与其他转录因子和辅因子协同工作。如我们之前所示，PU.1与p300/CBP和HDAC相互作用，p300/CBP起辅助激活作用，HDAC起辅助抑制作用。在本研究中，为了确定PU.1的修饰是否导致其在辅活化子和辅阻遏子之间的关联，我们研究了乙酰化是否调节PU.1的物理和功能活动。我们发现PU1在体内发生了乙酰化，当Ets结构域中可能的乙酰化基序发生突变时，其抑制活性降低。我们的数据提示，乙酰化可能调节红系细胞的生物学功能。

Transcription factor PU.1 is a hematopoietic master regulator and essential for the development of myeloid and B-cell lineages. PU.1 functions in concert with other transcription factors and cofactors. As we shown previously represented, PU.1 interact with both p300/cbp, which function as a coactivator and HDAC, which function as a corepressor. In this study, to determine whether modification of PU.1 is responsible for switching its association between coactivaor and corepressor, we examined whether acetylation regulates the physical and fnctional activities of PU.1. We found that PU.1 was acetylated in vivo and its repressor activity was reduced when the putative acetylation motif in ETS domain were mutated. Our data suggest that acetylation might regulate the biological functions in eryhroid cells.