Functional analysis of HIV-1 integrase that involves in various steps of viral replication

对猕猴嗜细胞HIV-1适应性突变的分析表明，这些突变集中在病毒基因组整合酶编码区的c端。即使是同义突变也被发现具有促进生长的作用。这种效应是由于Gag和Gag- pol蛋白的表达增强。对c端整合酶区域的广泛分析表明，同义突变对病毒复制产生积极和消极的影响。因此，很明显，这个整合酶区域调节病毒的复制能力。

Analysis of adaptive mutations in macaque cell-tropic HIV-1 has shown that these alterations cluster in the C-terminal portion of integrase-coding region in the viral genome. Even a synonymous mutation was found to have a growth-enhancing effect. This effect was due to the enhanced expression of Gag and Gag-Pol proteins. Extensive analysis of the C-terminal integrase region has revealed that synonymous mutations generate both positive and negative effects on viral replication. It is thus evident that this integrase region modulates viral replication ability.