New drug sensitivity test for leukemia mimicking bone marrow microenvironment in hypoxia

对于白血病的治疗，有必要通过精确的药敏试验对抗白血病药物进行评估。我们推测，模拟骨髓微环境的低氧培养系统比常氧培养系统更有利于评估细胞培养的效果。在这项研究中，我们发现低氧培养系统上调了白血病干细胞中HIF蛋白的表达，并影响了Notch、NF-kB和mTOR信号转导。我们为新的低氧药物敏感性试验奠定了基础。

For treatment of leukemia, it is necessary to assess antileukemia agent by precise drug sensitivity test. We assumed that cell culture system in hypoxia mimicking bone marrow microenvironment is useful for assess the effect than the system in normoxia. In this study, we found that hypoxia culture system upregurated HIF protein expression as well as affected Notch, NF-kB, and mTOR signaling in leukemia stem cells. We established a basis for a new drug sensitivity test in hypoxia.