The development and clinical application of the novel autoantibody for gastric cancer using proteomic analysis

我们构建了一个新的蛋白质组学分析系统，用于检测血清中的自身抗体。该系统由比较蛋白质印迹的第一筛选程序和随后的质量分析组成。首先，我们从四个低分化胃癌细胞系中提取蛋白质。以蛋白质提取物为抗原，采用免疫印迹法对胃癌患者和健康对照者血清进行分析。在这项分析中，我们检测到几个条带，并确定了30个蛋白质从这些带与质谱。其中，两个蛋白质被成功验证。这些蛋白被认为是低分化胃癌的新的生物标志物

We constructed a novel proteomic analysis system for the detecting of autoantibodies from sera. This system is composed of first screening procedure of comparative western blotting and subsequent mass analysis. At first, we extracted proteins from four poorly-differenciated gastric cancer cell lines. Using comprehensive western blotting in which protein extracts were used as antigen, we analysed sera from gastric cancer patients and healthy controls. In this analysis, we detected several bands, and identified thirty proteins from these bands with mass spectrometry. Among them, two proteins were successfully validated. These proteins were considered to be novel biomarkers for poorly-differnciated gastric cancer