The development and clinical application of the novel autoantibody for gastric cancer using proteomic analysis

蛋白质组学分析新型胃癌自身抗体的开发及临床应用

• 批准号: 22790522
• 负责人: UMEMURA Hiroshi
• 金额: $ 2.58万
• 依托单位: Okayama University (2011)Chiba University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790522/
• 关键词: プロテオーム; 癌; 臨床検査; 自己抗体; 腫瘍マーカー

We constructed a novel proteomic analysis system for the detecting of autoantibodies from sera. This system is composed of first screening procedure of comparative western blotting and subsequent mass analysis. At first, we extracted proteins from four poorly-differenciated gastric cancer cell lines. Using comprehensive western blotting in which protein extracts were used as antigen, we analysed sera from gastric cancer patients and healthy controls. In this analysis, we detected several bands, and identified thirty proteins from these bands with mass spectrometry. Among them, two proteins were successfully validated. These proteins were considered to be novel biomarkers for poorly-differnciated gastric cancer

我们构建了一个新的蛋白质组学分析系统，用于检测血清中的自身抗体。该系统由比较蛋白质印迹的第一筛选程序和随后的质量分析组成。首先，我们从四个低分化胃癌细胞系中提取蛋白质。以蛋白质提取物为抗原，采用免疫印迹法对胃癌患者和健康对照者血清进行分析。在这项分析中，我们检测到几个条带，并确定了30个蛋白质从这些带与质谱。其中，两个蛋白质被成功验证。这些蛋白被认为是低分化胃癌的新的生物标志物

项目成果

The measurement of a fibrinogen α C-chain 5.9 kDa fragment (FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution.

• DOI: 10.1016/j.cca.2011.02.029
• 发表时间: 2011-05
• 期刊: Clinica chimica acta; international journal of clinical chemistry
• 作者: K. Sogawa;Y. Kodera;K. Noda;Yusuke Ishizuka;M. Yamada;H. Umemura;K. Maruyama;T. Tomonaga;O. Yokosuka;F. Nomura

Identification of vitronectin as a novel serum marker for early breast cancer detection using a new proteomic approach

• DOI: 10.1007/s00432-010-0974-9
• 发表时间: 2011-07-01
• 期刊: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
• 影响因子: 3.6
• 作者: Kadowaki, Masami;Sangai, Takafumi;Miyazaki, Masaru
• 通讯作者: Miyazaki, Masaru

Serum Level of Fibrinogen A.ALPHA. Chain Fragment Increases in Chronic Thromboembolic Pulmonary Hypertension

纤维蛋白原 A.ALPHA 的血清水平。

• DOI: 10.1253/circj.cj-11-0045
• 发表时间: 2011
• 期刊: Circulation Journal
• 影响因子: 3.3
• 作者: Toshiaki Yano;et al
• 通讯作者: et al

Effects of humidity on the dried-droplet sample preparation for MALDI-TOF MS peptide profiling.

• DOI: 10.1016/j.cca.2010.08.010
• 发表时间: 2010-12
• 期刊: Clinica chimica acta; international journal of clinical chemistry
• 作者: H. Umemura;Y. Kodera;F. Nomura

Increased Serum Levels of Pigment Epithelium-Derived Factor by Excessive Alcohol Consumption-Detection and Identification by a Three-Step Serum Proteome Analysis

• DOI: 10.1111/j.1530-0277.2010.01336.x
• 发表时间: 2011-02-01
• 期刊: ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
• 影响因子: 3.2
• 作者: Sogawa, Kazuyuki;Kodera, Yoshio;Nomura, Fumio
• 通讯作者: Nomura, Fumio