The development and clinical application of the novel autoantibody for gastric cancer using proteomic analysis

我们构建了一种新的蛋白质组学分析系统，用于检测血清中的自身抗体。该系统由第一筛选程序比较免疫印迹和随后的质量分析组成。首先，我们从四种低分化胃癌细胞系中提取蛋白质。以蛋白质提取物为抗原，采用免疫印迹法对胃癌患者和健康对照者的血清进行分析。在这个分析中，我们检测了几个波段，并从这些波段中鉴定了30个蛋白质。其中，2个蛋白被成功验证。这些蛋白被认为是低分化胃癌的新生物标志物

We constructed a novel proteomic analysis system for the detecting of autoantibodies from sera. This system is composed of first screening procedure of comparative western blotting and subsequent mass analysis. At first, we extracted proteins from four poorly-differenciated gastric cancer cell lines. Using comprehensive western blotting in which protein extracts were used as antigen, we analysed sera from gastric cancer patients and healthy controls. In this analysis, we detected several bands, and identified thirty proteins from these bands with mass spectrometry. Among them, two proteins were successfully validated. These proteins were considered to be novel biomarkers for poorly-differnciated gastric cancer