Discovery of biomarkers for early colon cancer using a phospho-proteomic approach

使用磷酸化蛋白质组学技术（Phospho-iTRAQ）将结肠恶性肿瘤组织中的蛋白质磷酸化谱与结肠良性肿瘤和非肿瘤组织中的蛋白质磷酸化谱进行比较。在恶性肿瘤组织中，超过200个磷酸化位点的磷酸化水平发生了改变，这表明这些改变是结肠癌的候选者。为了验证这些候选人，磷酸-SI-SRM技术开发。使用这些蛋白质组学技术和生物信息学，我确定了结肠癌的候选生物标志物和参与结肠癌的信号蛋白。

The profile of protein phosphorylation in colon malignant tumor tissues were compared with those in colon benign tumor and non-tumor tissues using a phospho-proteomic technique(Phospho-iTRAQ). Phosphorylation levels at more than 200 phosphorylation sites were altered in the malignant tumor tissues, suggesting that these alterations are candidates for colon cancer. To validate these candidates, a phospho-SI-SRM technique was developed. Using these proteomic techniques and bioinformatics, I identified candidate biomarkers for colon cancer and signaling proteins involved in colon cancer.