Diagnostic systems of diffuse type gastric cancer using by epigenomic approach

通过甲基化CpG岛扩增和CpG岛微阵列（MCAM）分析，在肠型和弥漫型胃癌（GC）患者的胃粘膜中，RASGRF1甲基化显著升高。我们的数据表明，RASGRF1甲基化可能是一种有用的生物标志物，用于识别胃癌高风险个体。我们通过一项前瞻性研究评估了DNA甲基化作为异时性胃癌风险生物标志物的临床应用。我们的研究结果表明，背景胃粘膜中miR-34b/c的甲基化可能是预测异时性胃癌风险的有用标志物。

Using methylated-CpG island amplification coupled with CpG island microarray(MCAM) analysis, RASGRF1 methylation was significantly elevated in gastric mucosafrom patients with either intestinal or diffuse type gastric cancer (GC). Our datasuggest that RASGRF1 methylation could be a useful biomarker with which to identifyindividuals at high risk for GC.We evaluated the clinical utility of DNA methylation as a biomarker ofmetachronous GC risk by performing a prospective study. Our results suggest thatmethylation of miR-34b/c in background gastric mucosae may be a useful marker topredict metachronous GC risk.