Role of regulatory T cells and inflammatory macrophage/Kupffer cells in non-alcoholic steatohepatitis

调节性 T 细胞和炎症巨噬细胞/库普弗细胞在非酒精性脂肪性肝炎中的作用

• 批准号: 22790659
• 负责人: YOKOKAWA Junko
• 金额: $ 1.91万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790659/
• 关键词: 非アルコール性脂肪性肝炎; 制御性T細胞; NASH; Treg; クッパー細胞; 炎症性マクロファージ; 肝免疫

We investigated numbers and function of regulatory T cells(Tregs) in liver tissue in mouse model for metabolic syndrome. We found that the numbers of Tregs in KK-Ay mice were increased, whereas the numbers of Tregs in ob/ob mice and db/db mice were not increased compared to control C57Bl/6 mice. mRNA expression of the anti-inflammatory cytokines IL-4、IL-10, TGF-Βin Tregs were decreased in KK-Ay mice, ob/ob mice and db/db mice compared to control C57Bl/6 mice. Large numbers of CD8+effector T cells and inflammatory macrophage(M1) infiltrated liver tissue in ob/ob mice and db/db mice. Our findings suggest that the reduced anti-inflammatory effect of Tregs develop steatohepatitis.

我们研究了代谢综合征小鼠模型肝组织中调节性 T 细胞 (Treg) 的数量和功能。我们发现与对照C57Bl/6小鼠相比，KK-Ay小鼠中的Tregs数量增加，而ob/ob小鼠和db/db小鼠中的Tregs数量没有增加。与对照C57Bl/6小鼠相比，KK-Ay小鼠、ob/ob小鼠和db/db小鼠中Treg中抗炎细胞因子IL-4、IL-10、TGF-β的mRNA表达降低。 ob/ob小鼠和db/db小鼠的肝组织中大量CD8+效应T细胞和炎性巨噬细胞(M1)浸润。我们的研究结果表明，Tregs 的抗炎作用降低会导致脂肪性肝炎。