Development of a novel three-dimensional culture system for hepatitis viruses

新型肝炎病毒三维培养系统的开发

• 批准号: 22790664
• 负责人: MURAKAMI Shuko
• 金额: $ 2.58万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790664/
• 关键词: HBV; 肝細胞; 3 次元培養; キメラマウス; 肝炎; ウイルス; 感染症; 3次元培養; 細胞・組織

Investigation of hepatitis B virus (HBV) has been hampered by the absence of accessible in vitro modeling tools. The aim of this study is to design an in vitro model of HBV infection that would support long-term replication. Immortalized normal human hepatocytes, or primary human hepatocytes isolated from chimeric mice with human liver were cultured with three-dimensional (3D) culture system to obtain a tissue-like formation and exposed to HBV-infected serum samples. The HBV-DNA level in supernatants of the culture was higher than 10^5 copies/ml and continued to be detectable for >1 month. The extracellular HBsAg was detected during the culture period. The 3D culture system with hepatocytes provides an accessible in vitro tool to investigate HBV cell entry, replication and susceptibility to new anti-viral agents for multiple HBV genotypes/subgenotypes.

B型肝炎病毒（HBV）的研究一直受到缺乏体外建模工具的阻碍。本研究的目的是设计一种支持长期复制的HBV感染体外模型。用三维（3D）培养系统培养永生化的正常人肝细胞或从人肝嵌合小鼠中分离的原代人肝细胞，以获得组织样形成，并暴露于HBV感染的血清样品。培养物上清液中HBV-DNA水平高于10^5拷贝/ml，并持续可检测>1个月。在培养过程中检测到细胞外HBsAg。具有肝细胞的3D培养系统提供了一种可访问的体外工具，用于研究HBV细胞进入、复制和对多种HBV基因型/亚型的新抗病毒药物的敏感性。

项目成果

Mechanism of the dependence of hepatitis B virus genotype G on co-infection with other genotypes for viral replication

• DOI: 10.1111/jvh.12022
• 发表时间: 2013-04-01
• 期刊: JOURNAL OF VIRAL HEPATITIS
• 影响因子: 2.5
• 作者: Sakamoto, T.;Tanaka, Y.;Mizokami, M.
• 通讯作者: Mizokami, M.