Application of control method to inhibit pancreatic cancer cell growth and metastasis by the regulation of lumican glycosylation.

Lumcan在胰腺导管腺癌(PDAC)间质组织中的表达与肿瘤的侵袭性有关，且与预后不良有关。我们使用基因转染技术来检测从PDAC细胞分泌的LUMICAN的生物学作用。Lumcan基因转染的PDAC细胞能分泌70 kDa的Lumican蛋白，并具有激活的ERK信号通路。转染促进PDAC细胞生长，增加细胞与层粘连蛋白的黏附，抑制细胞侵袭，降低活性基质金属蛋白酶-9。使用siRNA下调Lumcan的表达会导致相反的细胞行为。因此，PDAC细胞分泌的70 kDa LUMICAN在细胞的生长和侵袭中起着重要的作用。

Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma(PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PDAC cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PDAC cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion.