Application of control method to inhibit pancreatic cancer cell growth and metastasis by the regulation of lumican glycosylation.

应用对照方法通过调节lumican糖基化抑制胰腺癌细胞生长和转移。

• 批准号: 22790675
• 负责人: YAMAMOTO Tetsushi
• 金额: $ 2.33万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790675/
• 关键词: ルミカン; 膵臓癌; 細胞増殖; 細胞浸潤; MMP-9; プロテオグリカン; 糖鎖解析

Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma(PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PDAC cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PDAC cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion.

Lumcan在胰腺导管腺癌(PDAC)间质组织中的表达与肿瘤的侵袭性有关，且与预后不良有关。我们使用基因转染技术来检测从PDAC细胞分泌的LUMICAN的生物学作用。Lumcan基因转染的PDAC细胞能分泌70 kDa的Lumican蛋白，并具有激活的ERK信号通路。转染促进PDAC细胞生长，增加细胞与层粘连蛋白的黏附，抑制细胞侵袭，降低活性基质金属蛋白酶-9。使用siRNA下调Lumcan的表达会导致相反的细胞行为。因此，PDAC细胞分泌的70 kDa LUMICAN在细胞的生长和侵袭中起着重要的作用。

项目成果

Neuroepithelial stem cell marker nestin regulates the migration, invasion and growth of human gliomas

• DOI: 10.3892/or.2011.1267
• 发表时间: 2011-07-01
• 期刊: ONCOLOGY REPORTS
• 影响因子: 4.2
• 作者: Ishiwata, Toshiyuki;Teduka, Kiyoshi;Naito, Zenya
• 通讯作者: Naito, Zenya

膵癌特異分泌型lumicanの細胞増殖への関与

胰腺癌特异性分泌的lumican参与细胞增殖

• 发表时间: 2011
• 作者: 山本哲志;松田陽子;川原清子;彭為霞;内藤善哉;石渡俊行
• 通讯作者: 石渡俊行

Glycoprotein form of lumican induced pancreatic cancer cell growth

糖蛋白形式的lumican诱导胰腺癌细胞生长

• 发表时间: 2010
• 作者: T.Ishiwata;K.Teduka;T.Yamamoto;K.Kawahara;Y.Matsuda;Z.Naito;山本哲志;山本哲志
• 通讯作者: 山本哲志

Comparison of Fixation Methods for Preservation of Morphology, RNAs, and Proteins From Paraffin-Embedded Human Cancer Cell-Implanted Mouse Models

• DOI: 10.1369/jhc.2010.957217
• 发表时间: 2011-01-01
• 期刊: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
• 影响因子: 3.2
• 作者: Matsuda, Yoko;Fujii, Takenori;Naito, Zenya
• 通讯作者: Naito, Zenya