Gene therapy for myocardial infarction by using lentivirus encoding kit ligand

慢病毒编码试剂盒配体对心肌梗死的基因治疗

• 批准号: 22790717
• 负责人: HIGUCHI Koji
• 金额: $ 2.33万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790717/
• 关键词: 分子心臓病態学; 心筋梗塞; 遺伝子治療

The purpose of this study is to evaluate the effect of kit ligand(KL) isoform against myocardial infarction(MI). The KL expression in transduced cells was confirmed by western blotting and flow cytometry. Endogenous KL-1, KL-2 and c-kit expression in infarcted mouse hearts were evaluated. We found that KL-2 expression in mouse hearts was diminished after MI. Therefor gene therapy using lentivirus encoding KL-2 may improve the prognosis after MI. We were supposed to finish in vivo experiments in this year, however we have difficulties in making large scale virus. We will continue to do in vivo experiments.

这项研究的目的是评估试剂配体（KL）同工型对心肌梗塞（MI）的影响。通过蛋白质印迹和流式细胞术证实了转导细胞中的KL表达。评估了梗塞小鼠心脏中内源性KL-1，KL-2和C-KIT表达。我们发现MI后小鼠心脏中的KL-2表达降低。因此，使用编码KL-2的慢病毒的基因疗法可以改善MI后的预后。我们应该在今年完成体内实验，但是我们很难生产大规模的病毒。我们将继续进行体内实验。

项目成果

急性心筋梗塞モデルマウスにおけるkit ligandおよびc-kit発現の検討

急性心肌梗死模型小鼠kit配体及c-kit表达的检测

• 发表时间: 2011
• 作者: 樋口公嗣;樋口公嗣
• 通讯作者: 樋口公嗣

タイトル:急性心筋梗塞モデルマウスにおけるkit ligandおよびc-kit発現の検討

标题：急性心肌梗死模型小鼠中kit配体和c-kit表达的检查

• 发表时间: 2011
• 作者: 樋口公嗣